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The black bodysuit, designed by Rubin Singer, was made out of python and iguana skin and leather.
"We would take a bet that if Beyoncé watched our video exposés, she'd probably not want to be seen again in anything made of snakes, lizards, rabbits or other animals who died painfully," said PETA in a statement. "Today's fashions are trending toward humane vegan options, and Beyoncé's Super Bowl outfit missed the mark on that score."
Beyonce has revealed that here performance at the Super Bowl took five months of performance.
"I'm feeling so proud, it was a really beautiful day," she told "Extra" after the performance. "All the hard work, five months of preparation, and it was really great." She was also thrilled about sharing the stage again with Michelle and Kelly, adding, "It really was a magnificent night for me and the girls."
Meanwhile according to estimates from the Nielsen company, her halftime show at Sunday's Super Bowl drew an average of 104 million viewers.
Last year's Madonna halftime show rang up 112.5 million viewers, which bested the previous high-point, Bruce Springsteen's 2009 performance, which got 95.7 million pairs of eyeballs.